CL-11-S1PR1
Name Sphingosine-1-phosphate Receptor 1 (S1PR1) ACTOneTM Stable Cell Line
Description This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR1. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR or a fluorescence microplate reader.
Application cAMP dependent assay for Gi-coupled human S1PR1; cell-based high-throughput screening of human S1PR1 inhibitors.
Size 1x 106 cells
Detection FlexStation or Microplate reader
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